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[Objective] To establish a method to determine the content of protocatechuic acid in CORTEX ACAN THOPANACIS.[Method] Content of protocatechuic acid in CORTEX ACAN THOPANACIS was determined by high performance liquid chromatography-four channel UV-Vis detection method.Chromatographic conditions were as follows:ODSC18 chromatographic column(250 mm×4.6 mm,5 μm) ,and buffer solution of methanol and sodium dihydrogen phosphate as the mobile phase(0.1 mol/L,15∶85,V/V,pH=2.5) .4 detection wavelengths were 245,260,275 and 290 nm with the quantitative detection wavelength of 260 nm.[Result] Protocatechuic acid had good linear relationship within the range of 0.5-6.0 mg/L(r=0.999 9) .The average recovery rate was 99.87% with RSD=2.11%.[Conclusion] The method was reliable,stable,and simple,and could be used for the content determination of protocatechuic acid in CORTEX ACAN THOPANACIS.
[Objective] To establish a method to determine the content of protocatechuic acid in CORTEX ACAN THOPANACIS. [Method] Content of protocatechuic acid in CORTEX ACAN THOPANACIS was determined by high performance liquid chromatography-four channel UV-Vis detection method. Chromatographic conditions were as ODSC18 chromatographic column (250 mm × 4.6 mm, 5 μm) and buffer solution of methanol and sodium dihydrogen phosphate as the mobile phase (0.1 mol / L, 15:85, V / V, pH = 2.5) [Result] Protocatechuic acid had good linear relationship within the range of 0.5-6.0 mg / L (r = 0.999 9). The average recovery rate was 99.87% with RSD = 2.11%. [Conclusion] The method was reliable, stable, and simple, and could be used for the content determination of protocatechuic acid in CORTEX ACAN THOPANACIS.