保存寄生虫的常规方法包括体外培养或动物转种,这些方法昂贵、繁琐,而且有一定风险,往往容易发生遗传漂变(geneticdrift)、人为差错或混淆。低温保存则可大大减少这些弊端。现有的低温保存方法已适用于多种阿米巴原虫。本文试图通过低温方法,保存经实验室连续培养的自由生活阿米巴原虫。 Conventional methods for preserving parasites include in vitro culture or animal transplants. These methods are expensive, cumbersome, and at risk, often prone to genetic drifting, human error, or confusion. Cryogenic preservation can greatly reduce these drawbacks. Existing cryopreservation methods have been applied to a wide range of amoebae. This article attempts to preserve the free-living amoeba that has been continuously cultured in the laboratory by a cryogenic method.