长期来,许多细胞生物学工作者希望能够在显微镜下直接看到组织和细胞中大分子物质的分布。用物理学(如光谱吸收)或组织化学方法虽能测定其在组织细胞中的存在,但反应专一性往往不如免疫学上的抗原一抗体反应。Coons 等首先利用荧光素来标记抗体,在肺炎球菌感染的小鼠组织切片内观察到肺炎球菌可 For a long time, many cell biologists hope to see the distribution of macromolecules in tissues and cells directly under the microscope. Although its presence in tissue cells can be measured by physical (eg, spectral absorption) or histochemical methods, the specificity of the reaction is often not as good as the immunological antigen-antibody reaction. Coons et al. First used fluorescein to label antibodies and pneumococci were observed in tissue sections of pneumococcal infected mice