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目的:研究Slug基因沉默对胰腺癌AsPC-l细胞增殖、凋亡的影响。方法:构建靶向抑制S1ug的siRNA表达载体,瞬时转染AsPC-1细胞。采用RT-PCR和Westernblot法检测细胞Slug蛋白,MTT检测细胞增殖,AnnexinV-FITC/PI了解细胞凋亡的变化。结果:成功构建了pGenesil-l-Slug-siRNA(pSlug-siRNA)和pCenesil-1-Neg-siRNA(pNeg-siRNA)表达质粒。pSlug-siRNA瞬时转染AsPC-1细胞72h后,细胞S1ug表达及生长受到明显抑制,细胞凋亡率明显增加。结论:Slug-siRNA抑制Slug表达,促进As-PC-1细胞凋亡并抑制细胞的增殖。
Objective: To investigate the effects of Slug gene silencing on proliferation and apoptosis of AsPC-1 cells. Methods: siRNA targeting siRNA targeting S1ug was constructed and transiently transfected into AsPC-1 cells. Slug protein was detected by RT-PCR and Western blot. MTT assay was used to detect cell proliferation. Annexin V-FITC / PI was used to detect the changes of cell apoptosis. RESULTS: Plasmid pGenesil-1-Slug-siRNA (pSlug-siRNA) and pCenesil-1-Neg-siRNA (pNeg-siRNA) were successfully constructed. Transfection of pSlug-siRNA with AsPC-1 cells for 72h resulted in significant inhibition of S1ug expression and cell apoptosis. Conclusion: Slug-siRNA can inhibit the expression of Slug, promote the apoptosis of As-PC-1 cells and inhibit the proliferation of cells.