为了研究肺癌中ｐ５３基因的作用及突变类型与不同致癌物的可能关系，用ＰＣＲ－ＳＳＣＰ核酸序列技术分析了原发非小细胞肺癌中ｐ５３基因的突变谱，结果发现１２８例中９４例发生突变，其中８６例为点突变，７例为缺失和插入突变，１例为拼接部位突变。点突变以Ｇ→Ｔ和Ｔ→Ｇ突变为主（６３．９％），Ｇ→Ａ和Ａ→Ｇ占２５．６％，这些结果提示ｐ５３基因突变与非小细胞肺癌发生有密切关系，并根据其突变特点分析了可能的环境致癌物。 In order to study the role of p53 gene and the possible relationship between mutation types and different carcinogens in lung cancer, the mutation spectrum of p53 gene in primary non-small cell lung cancer was analyzed by PCR-SSCP nucleic acid sequence technique. It was found that 94 out of 128 cases had mutations. Of these, 86 were point mutations, 7 were deletions and insertion mutations, and 1 was splice site mutations. The point mutations were dominated by G→T and T→G mutations (63.9%), and G→A and A→G accounted for 25.6%. These results suggest that the p53 gene mutation is closely related to the occurrence of non-small cell lung cancer, and Based on its mutation characteristics, possible environmental carcinogens were analyzed.