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1972-1973年春、秋季收集Ⅱ、Ⅲ、Ⅳ期雌鲤鱼脑垂体,用丙酮固定,应用凝胶分析圆盘电泳进行分离脑垂体的蛋白组分。每次电泳取21毫克经丙酮固定的脑垂体,在研缽中捣碎,随之加入1毫升蒸馏水,放在磁性搅拌器中,在室温条件下,搅拌1小时。脑垂体匀浆物离心15分钟(8,000转/分),用上清液作电泳分析,电泳结束后(电泳凝胶柱显色的前沿部分为64±1毫米),把每根电泳凝胶柱切成厚度为2.0-2.5毫米的薄片。一次实验取每根电泳凝胶柱上的同一个
The pituitary of female carps of stage Ⅱ, Ⅲ and Ⅳ were collected in the spring and autumn of 1973-1973. The pituitary gland was collected and fixed with acetone. The protein components of the pituitary gland were separated by gel analysis disk electrophoresis. Each electrophoresis takes 21 mg of acetone-fixed pituitary gland, mashed in a mortar, followed by the addition of 1 ml of distilled water, placed in a magnetic stirrer and stirred at room temperature for 1 hour. The pituitary homogenate was centrifuged for 15 minutes (8,000 rpm), and the supernatant was used for electrophoresis analysis. After the electrophoresis was completed (the front part of the gel electrophoresis gel column was 64 ± 1 mm), each gel electrophoresis gel column Cut into sheets with a thickness of 2.0-2.5 mm. One experiment takes the same one on each gel electrophoresis column