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探讨应用实时定量PCR方法在检测多发性骨髓瘤 (multiplemyeloma ,MM )患者微小残留病 (minimalresidualdisease ,MRD )中的作用。利用SYBRGreenI荧光染料 ,采用实时定量PCR方法 ,以IgH为标志 ,对 2 6例MM患者反应停治疗前后的IgH基因重排进行定量分析。结果发现 ,IgH (immunoglobulinheavychain )基因重排拷贝数 ,在反应停治疗完全缓解组、有效组前后分别为 (30 2 8± 6 5 5、 4 3± 2 4 )和 (74 6 7± 6 1 1、 6 1 9± 2 71 ) ,前后相比两者差异有显著性 (P <0 0 5 ) ,无效组 (6 1 5 3±1 5 2 7、 6 5 75± 1 2 2 7) ,前后差异无显著性 (P >0 0 5 ) ,并与患者骨髓浆细胞比例成正相关 (r=0 89,P <0 0 5 )。本实验表明 ,实时定量PCR对IgH基因重排的定量分析 ,可以监测MM患者体内MRD ,作为判断MM治疗疗效的一种检测方法 ,并对患者的预后判断有指导意义
To investigate the role of real-time PCR in the detection of minimal residual disease (MRD) in patients with multiple myeloma (MM). Quantitative analysis of IgH gene rearrangements in 26 patients with MM before and after treatment with SYBRGreenI fluorescent dye, real-time PCR and IgH was performed. The results showed that the copy number of IgH immunoglobulin heavy chain gene was (30 2 8 ± 6 5 5, 4 3 ± 2 4) and (74 6 7 ± 6 1 1) before and after treatment with complete remission , 6 1 9 ± 2 71). There was significant difference between before and after treatment (P <0.05), ineffective group (61 5 3 ± 1 57 27, 65 75 ± 1 2 2 7) There was no significant difference between the two groups (P> 0.05), which was positively correlated with the proportion of bone marrow plasma cells (r = 0 89, P 0 05). This experiment shows that quantitative analysis of real-time quantitative PCR for IgH gene rearrangement can monitor MRD in MM patients as a detection method to judge the therapeutic effect of MM, and is of guiding significance for the prognosis of patients