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间日疟原虫的研究由于体外培养技术的缺乏而进展迟缓。而体外连续性培养体系的建立由于间日疟原虫的本身特性及对侵入红细胞的特异性选择而受阻。近几年,研究用源自血色病患者血液和脐带血中的网织红细胞短期体外培养,可使间日疟原虫在体外生存达到一个月左右。目前,使用实验室源自脐带血造血干细胞分化的红系细胞能够不断的提供网织红细胞以及源于正常人肝组织的肝细胞株HC-04的新建立,完成了间日疟原虫体外连续性培养的目的。这里,我们着重介绍间日疟原虫的体外连续性培养技术。
Studies of Plasmodium vivax have been slow to progress due to lack of in vitro culture techniques. However, the establishment of an in vitro continuous culture system was hampered by the nature of Plasmodium vivax and the specific choice of invasive erythrocytes. In recent years, research using short-term reticulocyte culture in blood and umbilical cord blood derived from patients with hemochromatosis has allowed the Plasmodium vivax to survive in vitro for about a month. At present, erythroid cells derived from umbilical cord blood-derived hematopoietic stem cells in the laboratory are capable of continually providing a new establishment of reticulocyte and hepatocyte cell line HC-04 derived from normal human liver tissue, completing the in vitro continuity of P. vivax The purpose of training. Here, we highlight the in vitro continuous culture of Plasmodium vivax.