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目的探讨四磨汤对脾虚便秘小鼠肠道细菌丰度及功能基因的影响,为其疗效机制提供依据。方法采用番泻叶泄泻、饥饱失常和控制饮食等复合因素建立小鼠脾虚便秘模型,造模成功后,治疗组小鼠分别用四磨汤和枳术汤灌胃,模型组和正常组小鼠用无菌水灌胃。造模结束和治疗结束后分别提取小鼠肠道微生物总DNA,进行16SrDNA V4区测序。结果脾虚便秘造模降低了小鼠肠道细菌丰度,四磨汤治疗后,小鼠肠道细菌丰度整体水平上与模型组比较差异不显著。基于功能基因分析,造模降低了小鼠细胞信号传导、能量代谢、脂质代谢和一些辅酶、维生素代谢等的作用,提高了核苷酸代谢、碳水化合物代谢、氨基酸代谢和酶类等的水平。四磨汤口服液治疗后,小鼠的肠道细菌功能基因碳水化合物代谢、能量代谢、氨基酸代谢、核苷酸代谢、脂质代谢、多糖生物合成、遗传信息复制、翻译和跨膜运输等方面要显著高于其余各组。结论脾虚便秘小鼠降低了肠道细菌能量、糖类和脂质代谢,四磨汤可能通过提高肠道细菌能量和营养物质的代谢,提高遗传信息的复制、转录、翻译和跨膜转运水平来达到治疗脾虚便秘的效果。基于功能基因的角度分析为小鼠肠道细菌的改变提供了依据。
Objective To investigate the effects of Si mill decoction on intestinal bacterial abundance and functional genes in mice with constipation of spleen asthenia and to provide basis for its therapeutic mechanism. Methods The constipation models of mice with spleen deficiency caused by diarrhea in Senna, familial hunger and diet control were established. After the model was successfully established, the mice in the treatment group were administered with Sihuangtang and Zhizhu Tang, respectively. The model group and the normal group Mice with sterile water gavage. After the end of the model and the treatment, the intestinal microbial total DNA of the mice were respectively extracted and the 16S rDNA V4 region was sequenced. Results Spleen constipation modeling reduced intestinal bacterial abundance in mice, four mill soup treatment, the overall level of intestinal bacterial abundance in mice compared with the model group was no significant difference. Based on functional gene analysis, modeling reduced the cell signal transduction, energy metabolism, lipid metabolism and some coenzymes, vitamin metabolism, etc., increased the level of nucleotide metabolism, carbohydrate metabolism, amino acid metabolism and enzymes . Simvastatin oral administration of mice intestinal bacterial functional gene carbohydrate metabolism, energy metabolism, amino acid metabolism, nucleotide metabolism, lipid metabolism, polysaccharide biosynthesis, genetic information replication, translation and transmembrane transport and so on To be significantly higher than the rest of the groups. Conclusions Spleen-deficient constipation mice reduced the energy, carbohydrate and lipid metabolism of intestinal bacteria. Si-mill soup may improve the replication, transcription, translation and transmembrane transport of genetic information by increasing the energy and nutrient metabolism of intestinal bacteria Achieve the effect of constipation treatment of spleen. Based on functional gene analysis of the angle of gut bacteria in mice provided the basis for change.