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目的通过椎痛消胶囊药效学实验,评价炎性细胞在腰椎间盘突出症中的作用及椎痛消胶囊对其的影响。方法将健康雄性Wistar大鼠60只随机分为椎痛消胶囊高、中、低剂量组及阳性对照组、模型对照组、正常对照组,每组10只。采用自体髓核植入,模拟腰椎间盘突出症模型。各组连续给药30d,正常对照组、模型对照组灌服等量蒸馏水。对各组大鼠的背根神经节、神经根进行组织学观察,计数炎性细胞。结果模型组神经节、神经根均出现明显的炎性细胞漫润;椎痛消胶囊高、中、低剂量组及阳性对照组的背根神经节、神经根术后的炎性细胞漫润数目明显减少,与模型组相比差异有统计学意义(P<0.05,P<0.01,P<0.001),相应的神经根损害明显减轻。结论髓核组织介导的炎性细胞浸润及炎症反应在腰椎间盘突出症中起重要作用;椎痛消胶囊能有效减少炎性细胞浸润、减轻炎症反应和神经组织的损伤,对促进突出物的吸收有重要意义。
OBJECTIVE To evaluate the role of inflammatory cells in prolapse of lumbar intervertebral disc and the effect of Jiao Tong Xiao Capsule on the pharmacodynamic test of Jiao Tong Xiao Capsule. Methods Sixty healthy male Wistar rats were randomly divided into high, medium and low dosage groups of Zhitongxiao capsule and positive control group, model control group and normal control group, with 10 rats in each group. Autologous nucleus pulposus implantation, simulated lumbar disc herniation model. The rats in each group were administrated continuously for 30 days. The normal control group and the model control group were given the same amount of distilled water. The dorsal root ganglion and nerve root of each group were observed histologically and the inflammatory cells were counted. Results In the model group, obvious inflammatory cell infiltration was observed in the ganglion and the nerve root. The dorsal root ganglion, the number of infiltrative cells in the nerve root after operation in the high, medium and low dose group of Jiao Tong Xiao Capsule and the positive control group (P <0.05, P <0.01, P <0.001), and the corresponding nerve root lesions were significantly reduced. Conclusion Nucleus pulposus tissue-mediated inflammatory cell infiltration and inflammatory reaction play an important role in lumbar disc herniation. Jiao Tongxiao capsule can effectively reduce inflammatory cell infiltration, reduce inflammation and damage of nerve tissue, Absorption is important.