论文部分内容阅读
目的:观察热休克蛋白90(HSP90)抑制剂对前列腺癌PC-3M细胞增殖活性、细胞周期和凋亡的影响,初步探讨其作用机制。方法:采用四甲基偶氮唑盐(MTT)比色法检测不同浓度的HSP90抑制剂对PC-3M细胞增殖的影响;采用细胞免疫化学测定HSP90抑制剂对PC-3M细胞HER-2表达的影响;采用流式细胞分析术(FCM)检测细胞凋亡和细胞周期。结果:高浓度(500 nmol/L)HSP90抑制剂可杀伤PC-3M细胞,低浓度(10、50、125、250 nmol/L)条件下则主要发挥生长抑制作用。HSP90抑制剂可使PC-3M细胞HER-2表达水平降低,并在500 nmol/L浓度时诱导细胞凋亡。结论:HSP90抑制剂较为明显地抑制PC-3M细胞生长,并在高浓度时杀伤癌细胞;其作用机制可能与下调HER-2的表达有关。
OBJECTIVE: To observe the effects of heat shock protein 90 (HSP90) inhibitor on the proliferation, cell cycle and apoptosis of prostate cancer PC-3M cells and to explore its possible mechanism. Methods: MTT assay was used to detect the effect of different concentration of HSP90 inhibitor on the proliferation of PC-3M cells. Immunocytochemistry was used to measure the expression of HER-2 in PC-3M cells by HSP90 inhibitor The apoptosis and cell cycle were detected by flow cytometry (FCM). RESULTS: HSP90 inhibitor at high concentration (500 nmol / L) could kill PC-3M cells and inhibit the growth of PC-3M cells at low concentration (10, 50, 125 and 250 nmol / L) HSP90 inhibitors reduced HER-2 expression in PC-3M cells and induced apoptosis at 500 nmol / L. Conclusion: The HSP90 inhibitor can significantly inhibit the growth of PC-3M cells and kill the cancer cells at high concentration. Its mechanism may be related to the down-regulation of HER-2 expression.