目的 :建立高效液相色谱法测定感冒清热颗粒中葛根素含量。方法 :采用SuplcosilC16色谱柱 (15 0mm× 4 6mm ,5μm) ,甲醇 -水 (2 7∶73)为流动相 ,流速 1 0mL·min-1,检测波长为 2 48nm。结果 :葛根素在 0 2～ 2 0 0 μg·mL-1范围内 ,峰面积与其浓度线性关系良好 (r =0 9999) ,平均回收率为 10 1 7% ,RSD为 1 3 % (n =3)。结论 :本法简便、准确、重复性好 ,可用于该制剂质量控制 Objective : To establish a HPLC method for the determination of puerarin in Ganmaoqing heat granules. METHODS: A Suplcosil C16 column (15 mm × 46 mm, 5 μm) was used. Methanol-water (27:73) was used as the mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was 2 48 nm. RESULTS: The puerarin ranged from 0.2 to 200 μg·mL-1. The linear relationship between the peak area and the concentration was good (r = 0 9999). The average recovery was 101.7%, and the RSD was 13% (n = 3). Conclusion : This method is simple, accurate, and reproducible. It can be used for the quality control of the preparation.