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建立HPLC法同时测定通宣理肺颗粒中甘草苷、橙皮苷、迷迭香酸3种成分的含量。方法:采用Thermo Syncronis C_(18)(250mm×4.6mm,5μm)色谱柱;乙腈-0.01%磷酸水溶液为流动相,梯度洗脱;柱温27℃;检测波长278nm;流速0.8mL·min~(-1)。结果:甘草苷、橙皮苷、迷迭香酸进样量分别在0.034~0.681μg(r=0.999 9)、0.256~5.115μg(r=0.999 8)、0.022~0.443μg(r=0.999 8)范围内线性关系良好,加样回收率分别为98.8%、99.3%、99.1%,RSD分别为1.01%、0.63%、1.33%。结论:该方法简便、准确、稳定,可为通宣理肺颗粒的质量控制提供参考。
A HPLC method was established for the simultaneous determination of three components of licorice glycosides, hesperidin and rosmarinic acid in Tongxuanlifei granule. Methods: The column of Thermo Syncronis C_ (18) (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile and 0.01% phosphoric acid. The column temperature was 27 ℃, the detection wavelength was 278 nm, the flow rate was 0.8 mL · min ~ -1). Results: The injection volume of glycyrrhizin, hesperidin and rosmarinic acid were 0.034 ~ 0.681μg (r = 0.999 9), 0.256 ~ 5.115μg (r = 0.999 8) and 0.022 ~ 0.443μg The range of linearity was good. The recoveries were 98.8%, 99.3% and 99.1%, respectively, with RSDs of 1.01%, 0.63% and 1.33%, respectively. Conclusion: The method is simple, accurate and stable, which can provide reference for the quality control of Xuanfei Pulmonary Particles.