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为探究石油炼化废水对ANAMMOX过程中脱氮性能的影响,对AAOB细菌混培物进行驯化试验,并利用MPN-PCR技术对驯化前后的主要菌群进行计数。结果表明,石油炼化废水添加比例为50%时,NH_4~+-N和NO_2~--N的平均去除负荷分别为3.535、5.442 kg/(m~3·d),平均理论COD差值为16.67 mg/L;石油炼化废水添加比例为100%时,NH_4~+-N和NO_2~--N的平均去除负荷分别为未添加的17.4%和62.5%。AAOB细菌混培物对石油炼化废水存在一定的适应性,当废水添加比例为50%时增加了系统的稳定性和有效脱氮效果,驯化前后AAOB和DNB菌群数目变化较大,并且对菌群生化活性的影响方式不同;尽管驯化过程中优势菌群逐渐发生变化,但二者的协同脱氮作用一直存在。
In order to explore the impact of petroleum refining wastewater on the denitrification performance of ANAMMOX process, AAOB bacterial mixture was domesticated and MPN-PCR technique was used to count the main bacterial flora before and after domestication. The results showed that the average removal load of NH_4 ~ + -N and NO_2 ~ -N was 3.535 and 5.442 kg / (m ~ 3 · d) respectively when the proportion of petroleum refinery wastewater was 50%. The average COD difference was 16.67 mg / L, respectively. When the proportion of petroleum refinery wastewater was 100%, the average removal load of NH 4 + -N and NO 2 - -N was 17.4% and 62.5% respectively. AAOB bacteria mixture has certain adaptability to petroleum refinery wastewater. When the proportion of wastewater added is 50%, the system stability and effective denitrification effect are increased. The number of AAOB and DNB flora before and after domestication is greatly changed, The biochemical activity of the flora varied in different ways. Although the dominant microflora gradually changed during domestication, the synergistic effect of denitrification existed.