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目的:研究包裹在PLGA微球中的多糖纳米颗粒在保护蛋白稳定性和改善药物体外释放行为方面的作用。方法:将模型药物BSA用低温诱导相分离方法担载于多糖纳米颗粒之中,后将其用水包油包固复乳法包裹于PLGA微球内。应用体积排阻色谱(SEC-HPLC)和红外色谱(FTIR)表征蛋白的稳定性,而且也研究了样品的体外释放行为。结果:这种方法能够很好的保护蛋白的稳定性,保持蛋白结构在制备过程中不会改变,而且改善了体外释放行为,减少了突释。结论:多糖纳米颗粒结合PLGA微球能够提供一种有效的解决蛋白控释的途径。
AIM: To investigate the effect of polysaccharide nanoparticles encapsulated in PLGA microspheres on the stability of protein and the drug release in vitro. Methods: The model drug BSA was loaded on the polysaccharide nanoparticles by low temperature induction phase separation method, and then encapsulated in PLGA microspheres by oil-in-water-encapsulation and resuscitation methods. The stability of the protein was characterized by size exclusion chromatography (SEC-HPLC) and infrared spectroscopy (FTIR), and the in vitro release behavior of the sample was also investigated. Results: This method can well protect the stability of protein, keep the protein structure unchanged in the process of preparation, and improve the in vitro release behavior and reduce the burst release. Conclusion: Polysaccharide nanoparticles combined with PLGA microspheres can provide an effective way to solve the protein controlled release.