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本实验用胶原酶消化法分离新生大鼠胰岛,培养和Ficoll密度梯度离心纯化胰岛。用海藻酸-聚赖氨酸-海藻酸生物膜包裹胰岛并进行体外胰岛素分泌功能测定和体内异种移植实验。10只链脲霉素诱导的糖尿病小鼠腹腔内移植微囊化胰岛3天后,小鼠血糖降至正常,体重逐步增加,糖尿病得到纠正。维持最长者达130天,平均85.2天。明显优于单纯胰岛移植组,其功能维持不足9天。肾脏病理组织染色和透射电镜显示早期微囊化胰岛移植确能防止糖尿病并发症的发生。
In this experiment, the islets of neonatal rat were isolated by collagenase digestion, and the islets were purified by Ficoll density gradient centrifugation. The islets were encapsulated with alginate-polylysine-alginate biofilms and the in vitro insulin secretion assay and in vivo xenograft assay were performed. After three days of transplantation of microencapsulated islets in 10 streptozotocin-induced diabetic mice, blood glucose in the mice was reduced to normal, body weight gradually increased, and diabetes was corrected. Maintain the longest up to 130 days, an average of 85.2 days. Obviously better than the simple islet transplantation group, its function to maintain less than 9 days. Renal pathological staining and transmission electron microscopy showed that early microencapsulated islet transplantation can prevent the occurrence of diabetic complications.