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目的:比较橄榄苦苷与其苷元羟基酪醇的大鼠在体胃肠吸收差异,分析橄榄苦苷在大鼠体内的代谢产物,为女贞子酒蒸增效机制研究提供科学依据。方法:采用大鼠在体胃肠灌流模型,以HPLC测定给药后不同时间点大鼠胃与小肠灌流流出液中橄榄苦苷和羟基酪醇的含量,流动相乙腈(A)-0.1%甲酸水溶液(B)梯度洗脱(0~10 min,10%~20%A;10~25min,20%~35%A),检测波长240 nm。计算二者的吸收速率常数和累计吸收率;通过已知橄榄苦苷、羟基酪醇对照品定位,分析橄榄苦苷在大鼠胃和小肠中的代谢产物。结果:羟基酪醇在大鼠胃和小肠中的吸收速率常数和累计吸收率均显著高于橄榄苦苷;在橄榄苦苷的大鼠胃和小肠代谢物中均检出羟基酪醇。结论:羟基酪醇较橄榄苦苷更利于机体吸收,橄榄苦苷在大鼠胃和小肠中均可代谢为羟基酪醇。
OBJECTIVE: To compare the differences in body and gastrointestinal absorption between oleuropein and its aglucone hydroxytyrosol in rats, and to analyze the metabolites of oleuropein in rats. Methods: The content of oleuropein and hydroxytyrosol in stomach and small intestine perfusate of rats were determined by HPLC in vivo and in vivo after gastrointestinal perfusion. The mobile phase of acetonitrile (A) - 0.1% formic acid The aqueous solution (B) gradient elution (0 ~ 10 min, 10% ~ 20% A; 10 ~ 25min, 20% ~ 35% A), detection wavelength 240 nm. Calculate the absorption rate constant and cumulative absorption rate of both; Through the known oleuropein, hydroxytyrosol reference substance positioning, analysis of oleuropein metabolites in rat stomach and small intestine. Results: The absorption rate constant and cumulative absorption rate of hydroxytyrosol in stomach and small intestine of rats were significantly higher than those of oleuropein. Hydroxytyrosol was detected in the stomach and small intestine metabolites of oleuropein. CONCLUSION: Hydroxytyrosol is more beneficial to the body than oleuropein, and oleuropein can be metabolized to hydroxytyrosol in the stomach and small intestine of rats.