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目的通过对传统培养法和PCR法在假丝酵母菌感染检出率的比较,拟探索一种能够早期、快速、高效检测头颈部放疗患者假丝酵母菌感染的方法。方法收集120名头颈部放疗患者唾液,分别应用假丝酵母菌显色培养基(CHROMagar)进行分离、培养和鉴定;同时提取基因组DNA,通过假丝酵母菌通用引物、特异性引物、改良引物进行PCR扩增,结果与假丝酵母菌表型进行对比。结果与传统培养法相比,PCR法检出率更高(χ2=47.672,P=0.000);改良特异性引物D扩增的检出率为77%,高于通用引物B(χ2=7.702,P=0.006)和特异性引物C(χ2=12.522,P=0.001)。结论本研究证实PCR技术耗时短,阳性检出率高,可用于头颈部肿瘤放疗患者假丝酵母菌感染的快速检测。
OBJECTIVE: To compare the detection rate of Candida infection between traditional culture method and PCR method and to find out a new method to detect Candida infection in patients with head and neck radiotherapy in an early, rapid and efficient way. Methods Saliva samples of 120 patients with head and neck radiotherapy were collected and separated by CHROMagar. The genomic DNA was extracted and purified by Candida universal primers, specific primers and modified primers PCR amplification, the results compared with the Candida phenotype. Results Compared with the traditional culture method, the detection rate of PCR was higher (χ2 = 47.672, P = 0.000). The detection rate of modified primer D was 77%, higher than that of universal primer B (χ2 = 7.702, P = 0.006) and specific primer C (χ2 = 12.522, P = 0.001). Conclusion This study confirmed that PCR technology is short time-consuming and has a high positive detection rate. It can be used for rapid detection of Candida infection in patients with head and neck cancer radiotherapy.