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目的:探讨还原型谷胱甘肽(GSH)对大鼠力竭运动后自由基和心肌细胞凋亡的影响。方法:30只SD大鼠随机分为对照组、力竭组和GSH组,每组10只。对照组大鼠安静状态下饲养,腹腔注射生理盐水1周后取样;力竭组大鼠腹腔注射生理盐水1周后进行一次性力竭运动,运动后即刻取样;GSH组大鼠给予GSH腹腔注射1周后,进行一次性力竭运动,运动后即刻取样。检测各组大鼠血清及心肌丙二醛(MDA)含量和心肌超氧化物歧化酶(SOD)活性,并采用TUNEL法检测心肌细胞凋亡率。结果:力竭组大鼠心肌SOD活性显著低于对照组和GSH组(P<0.05);力竭组大鼠血清和心肌MDA含量均显著高于对照组和GSH组(P<0.05)。力竭组大鼠心肌细胞凋亡率均显著高于对照组和GSH组(P<0.05)。结论:氧自由基生成增加和(或)SOD活性降低导致大鼠力竭运动后心肌细胞凋亡增加;GSH可减轻力竭运动后大鼠心肌细胞凋亡程度。
Objective: To investigate the effect of reduced glutathione (GSH) on the free radical and cardiomyocyte apoptosis after exhaustive exercise in rats. Methods: Thirty SD rats were randomly divided into control group, exhaustive group and GSH group, with 10 rats in each group. Rats in the control group were housed in a quiet state for 1 week after the intraperitoneal injection of normal saline. The rats in the exhaustive group were intraperitoneally injected with normal saline for one week and then subjected to a one-time exhaustive exercise. The rats in the GSH group were intraperitoneally injected with GSH After 1 week, a one-time exhaustive exercise, sampling immediately after exercise. Serum and myocardial MDA levels and myocardial SOD activity were measured in each group. Cardiomyocyte apoptosis was detected by TUNEL method. Results: The SOD activity in cardiac muscle of rats in exhaustive group was significantly lower than that in control group and GSH group (P <0.05). MDA content in serum and myocardium of rats in exhausted group was significantly higher than that in control group and GSH group (P <0.05). The apoptosis rate of cardiomyocytes in exhaustive group was significantly higher than that in control group and GSH group (P <0.05). CONCLUSION: Increased production of oxygen free radicals and / or decreased SOD activity lead to increased cardiomyocyte apoptosis after exhaustive exercise in rats. GSH can attenuate cardiomyocyte apoptosis in rats after exhaustive exercise.