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不同基因型小麦幼穗愈伤组织诱导能力及绿苗分化能力差异很大。根据原初愈伤组织状态实验材料划分为Ⅰ、Ⅱ、Ⅲ 3种类型。Ⅱ型愈伤组织状态胚性较好 ;不同浓度ABA和幼穗提取液可使Ⅰ型和Ⅲ型愈伤组织向Ⅱ型发展 ,0 5～ 1 0mg·L-1的ABA适于Ⅰ型向Ⅱ型的转化 ,2 0～ 2 5ml·L 1的幼穗提取液适于Ⅲ型向Ⅱ型的转化 ;诱导培养基中不宜添加KT ,0 5mg·L-1的KT表现抑制作用。
The callus induction ability and the ability of green shoot differentiation of different genotypes of wheat differed greatly. According to the initial callus state experimental materials are divided into Ⅰ, Ⅱ, Ⅲ 3 types. The callus of type Ⅱ callus had better embryogenicity. The callus of type Ⅰ and type Ⅲ developed to type Ⅱ with different concentrations of ABA and young ear extract. The ABA of 0 5 ~ 100 mg · L -1 was suitable for type Ⅰ Ⅱ type of transformation, 20 ~ 25ml · L 1 young spike extract suitable for type Ⅲ to Ⅱ type transformation; induction medium should not be added KT, 0 5mg · L-1 KT showed inhibition.