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用鼻咽癌细胞株 CNE-1作抗原,免疫 Balb/c 小鼠,取脾细胞与骨髓瘤细胞融合,获得13株杂交瘤细胞系,用酶联免疫吸附试验,杂交瘤 H_(13)分泌的单克隆抗体显较好的特异性,用间接免疫荧光检查,这些抗体作用部位为癌细胞膜上的肿瘤相关抗原。
The nasopharyngeal carcinoma cell line CNE-1 was used as an antigen to immunize Balb/c mice. The splenocytes were fused with myeloma cells to obtain 13 hybridoma cell lines. The hybridoma H_(13) was secreted by enzyme-linked immunosorbent assay. The monoclonal antibodies showed good specificity and were examined by indirect immunofluorescence. The sites of these antibodies were tumor-associated antigens on the cancer cell membrane.