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目的:了解南京地区丙型肝炎病毒(HCV) 感染及乙型肝炎病毒(HBV) 抗原亚型分布情况。方法:对1993 ～1995 年住院肝病患者2 157 例,应用RT- PCR、HCV 进行基因型、阳性克隆序列分析及HBV亚型的检测。结果:HCVRNA 阳性率为8.7 % 。对100 例HCV 基因型分析:Ⅱ型占91% ,Ⅲ型占6% 。HBV亚型分析:adr 占55.6% ,adw 占38.4% 。2 例HCV 阳性克隆均为Ⅲ型,与日本株(HC- J5 、HC-J6)密切相关。结论:中国南京的HCV可能是近代从日本传入的,HBV亚型以adr、adw 为主
Objective: To understand the distribution of hepatitis C virus (HCV) and hepatitis B virus (HBV) subtypes in Nanjing area. Methods: A total of 2 157 inpatients with liver disease from 1993 to 1995 were genotyped, positive cloned sequence analysis and HBV subtypes were detected by RT-PCR and HCV. Results: The positive rate of HCVRNA was 8.7%. Analysis of 100 HCV genotypes: type Ⅱ accounted for 91%, type Ⅲ accounted for 6%. HBV subtype analysis: adr accounted for 55.6%, adw accounted for 38.4%. The two HCV positive clones were all type Ⅲ, closely related to Japanese strains (HC-J5, HC-J6). Conclusion: HCV in Nanjing, China may be introduced from Japan in modern times. HBV subtypes are mainly adr and adw