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本文检测了用基因芯片筛选出水稻种子被低能N+辐照后引起的差异表达的ABA代谢和信号途径相关基因。结果显示,与ABA合成相关的ZDS、Lyc-β、ZEP、NCED、SDR这五种酶的基因表达量均为上调;受ABA调控的的H+-ATPase、NR、Rubisco的基因表达变化显著;ABA依赖的逆境应答蛋白DREB和ASR的表达量上调;受ABA信号转导调控的蛋白LEA的表达量下调,GAD和P5CS的表达量上调。这些结果表明,6×1017N+·cm-2剂量的辐照可能促进了ABA的合成和幼苗气孔的开放,同时促进了ABA信号系统并激活或抑制了一些相关基因的表达。
In this study, genes related to ABA metabolism and signal pathway that were differentially expressed in rice seeds after they were irradiated by low energy N + were detected by microarray. The results showed that the gene expression of ZDS, Lyc-β, ZEP, NCED and SDR related to ABA synthesis were all up-regulated. The ABA-regulated gene expression of H + -ATPase, NR and Rubisco changed significantly. Dependent stress response proteins DREB and ASR were up-regulated; the expression of LEA regulated by ABA signal transduction was down-regulated and the expression of GAD and P5CS was up-regulated. These results indicated that the irradiation of 6 × 1017N + · cm-2 could promote the synthesis of ABA and the stomatal opening of seedlings, promoted the ABA signaling system and activated or inhibited the expression of some related genes.