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环境因素影响昆虫两性间的化学通讯,也影响性信息素技术的田间防治效果.本文探讨了温度对二化螟雌蛾性信息素产生以及雄蛾对性信息素触角电位反应的影响,以期为田间二化螟的性信息素防治提供指导.在二化螟蛹期和成虫期进行不同温度处理(15、20、25、30和35℃),然后利用气相色谱仪(GC)分析雌蛾性腺内各性信息素组分的含量及比例,同时利用触角电位仪(EAG)测定雄蛾对性信息素组分的电生理反应.结果表明:25℃处理中雌蛾性腺内3个性信息素组分(Z9-16:Ald、Z11-16:Ald和Z13-18:Ald)的含量均显著高于其他温度处理(15、20和30℃),且25℃处理中Z13-18:Ald的相对比例也显著低于其他温度处理.就雄蛾对性信息素的敏感性而言,对3种性信息素单一组分及特定比例混合物的EAG反应在15~25℃间没有显著差异,但在25~35℃间(Z13-18:Ald在30~35℃间)随温度升高呈下降趋势,且30℃较25℃显著降低,35℃较30℃又显著降低.综合分析认为,二化螟性信息素通讯的适宜温度为20~25℃,温度过高或过低均不利于二化螟两性间的正常化学通讯.研究结果为二化螟性信息素防治技术的合理应用及极端温度条件下害虫种群发生的预测预报,提供了重要参考.
Environmental factors affect the insecticidal chemical communication between the sexes, but also affect the field of pheromone prevention and control effect.In this paper, the effect of temperature on sexual pheromone production of the female sex pheroplankton and the antennal potential response of sex pheromone were studied in this paper, Field Pneumoconiosis Sex Pheromone Prevention Guidance The Pseudoperis spp. Pupae and adult stages were subjected to different temperature treatments (15, 20, 25, 30 and 35 ℃), and then analyzed by gas chromatography (GC) The contents and proportions of the sex pheromone components in the female sex pheromone and the electrophysiological responses of the sex pheromone components to the male sex pheromone were measured by the EAG method.The results showed that the three sex pheromone groups (Z9-16: Ald, Z11-16: Ald and Z13-18: Ald) were significantly higher than the other temperature treatments (15, 20 and 30 ° C) and the relative Z13-18: Ald at 25 ° C The proportion was also significantly lower than other temperature treatments.For the male sex pheromone sensitivity, the sex pheromone single component and a specific mixture of EAG response between 15 ~ 25 ℃ no significant difference, but in the Between 25 and 35 ℃ (Z13-18: Ald between 30 and 35 ℃) showed a decreasing trend with the increase of temperature, and 30 ℃ was significantly higher than 25 ℃ And decreased significantly at 35 ℃ and 30 ℃ .A comprehensive analysis showed that the optimum temperature of sex pheromone communication was 20 ~ 25 ℃, the temperature is too high or too low are not conducive to the chemical communication between the sexes of the STI. The results provide an important reference for the rational application of the sex pheromone prevention and control technology and the prediction of pest population occurrence under the extreme temperature conditions.