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目的探讨经切割穹隆海马伞侧海马提取液诱导而激活的海马放射状胶质细胞的基因表达变化。方法将分离、纯化后的海马放射状胶质细胞分别加入正常侧和切割穹隆海马伞侧海马提取液,7d后利用免疫荧光法检测两组细胞的数量和生长情况;利用基因芯片分析细胞内基因表达情况;利用实时定量PCR分析神经干细胞向神经元分化的相关基因Rest和Runx1t1的转录情况。结果与正常组相比,切割组放射状胶质细胞的数量明显增多,胞体增大,突起增粗变长。基因芯片结果显示,在所检测的709个基因中,切割组中10个基因表达明显上调,10个基因明显下调;实时定量PCR结果表明,切割组Rest和Runx1t1的转录水平均显著高于正常组(P<0.05)。结论切割穹隆海马伞海马提取液可诱导海马放射状胶质细胞的激活,提高Rest和Runx1t1基因转录水平。
Objective To investigate the gene expression changes of hippocampal radial glial cells induced by activated hippocampal extracts of the hippocampus in rats. Methods The isolated and purified hippocampal radial glial cells were added into the hippocampus of the hippocampus on the normal side and the hippocampus, respectively. After 7 days, the numbers and growth of the cells in the two groups were detected by immunofluorescence. The expression of intracellular gene The real-time quantitative PCR was used to analyze the transcriptional status of Rest and Runx1t1 genes involved in the differentiation of neural stem cells into neurons. Results Compared with the normal group, the number of radial glial cells in the cutting group increased significantly, the cell bodies increased, and the protrusions grew thicker and longer. The results of gene microarray showed that among the 709 genes examined, 10 genes were significantly up-regulated and 10 genes were significantly down-regulated in the cleaved group. Real-time quantitative PCR results showed that the transcriptional levels of Rest and Runx1t1 in the cleaved group were significantly higher than those in the normal group (P <0.05). Conclusion Incision hippocampus hippocampus hippocampus extract can induce activation of hippocampal radial glial cells and increase Rest and Runx1t1 gene transcription levels.