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目的 探讨RGH患者血清细胞免疫功能的变化。方法 采用PHA诱导淋巴细胞增殖 ,观测淋巴细胞诱导后的增殖能力 ,并测定细胞因子IL 2和IFN γ血清浓度的变化 ;用LDH释放法测定NK和LAK细胞的杀伤活性 ,对结果进行统计学处理。结果 与对照组比较 ,RGH患者对PHA诱导的淋巴细胞增殖能力显著下降 (P <0 .0 5 ) ;血清IL 2和IFN γ水平也显著降低 (P <0 .0 1) ;NK和LAK细胞的杀伤活性亦明显降低 (P <0 .0 1)。结论 RGH患者细胞免疫功能紊乱是其易复发的免疫学发病机制。
Objective To investigate the changes of serum cellular immune function in patients with RGH. Methods The proliferation of lymphocytes was induced by PHA and the proliferation of lymphocytes was observed. The changes of cytokines IL 2 and IFN γ were measured. The killing activity of NK and LAK cells was measured by LDH release assay, and the results were statistically analyzed . Results Compared with the control group, the ability of RGH patients to induce PHA-induced lymphocyte proliferation was significantly decreased (P <0.05); the levels of serum IL-2 and IFN-γ were also significantly decreased (P <0.01); NK and LAK cells The killing activity was also significantly lower (P <0.01). Conclusion The cellular immune dysfunction in patients with RGH is an immunological mechanism of relapse.