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[目的]萝卜细胞质雄性不育(OguCMS)是甘蓝型油菜杂种优势利用理想途径,但由于恢复基因仅存在于萝卜基因组难以直接利用,转育萝卜恢复基因是实现甘蓝型油菜OguCMS应用突破的关键。[方法]本研究以萝-蓝(Rap hanobrassica,2n=58)为恢复基因供体材料,利用嫁接克服远缘杂交生殖障碍,并对获得的属间杂种通过甘蓝型油菜性状、自(异)交结实性状以及恢复率稳定性状等强化筛选,育成了一份甘蓝型油菜OguCMS纯合恢复材料CLR650。[结果]该恢复材料体细胞染色体数目在38～40条之间,减数分裂过程中部分细胞存在染色体落后及形成染色体桥等异常现象,但细胞异常对正常花粉最终形成没有明显影响,恢复株自交、测交均为100%可育。以基于OguCMS恢复基因(Rf0)设计的特异分子标记进行检测,证明该材料存在OguCMS恢复基因。以与之连锁其它萝卜分子标记分析比较,表明其萝卜片段长度短于国外同类恢复系R113。[结论]CLR650的育成为甘蓝型油菜OguCMS创制了新型恢复材料。
[Objective] The research aimed to utilize heterosis of rapeseed (Brassica napus L.) as an ideal pathway for heterosis utilization of radish. [Objective] Radish cytoplasmic male sterility (OguCMS) is the ideal way to utilize heterosis of Brassica napus. [Method] Raphalobacterium saponins (2n = 58) was used as the donor gene of the restorer gene, and grafting was used to overcome the distant hybridization reproductive failure. The obtained intergeneric hybrids were characterized by self - Cross-solid trait and recovery rate stability traits such as strengthening screening, bred a Brassica napus OguCMS homozygous recovery material CLR650. [Result] The numbers of somatic chromosomes in the restoration material ranged from 38 to 40, and some cells in the recovery process had some chromosome abnormalities, such as chromosomal backwardness and chromosome bridge formation. However, abnormal cells did not affect the final formation of normal pollen. Self-self, cross test are 100% fertile. The detection of specific molecular markers based on OguCMS restorer gene (Rf0) proved that OguCMS restorer gene existed in this material. The molecular markers of other radish linkage analysis showed that the length of radish segment was shorter than that of R113. [Conclusion] The breeding of CLR650 was a new recovery material developed by OguCMS in Brassica napus.