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为研究‘大久保’资源的多样性,以不同来源的9份‘大久保’种质和26份其它桃种质为试材,利用SSR标记,进行了亲缘关系分析。PCR扩增结果表明,从44对引物中筛选出的13对SSR引物共扩增出93个等位基因,其中多态性为91条,达97.85%,平均引物多态性条带数目为7条。聚类结果表明:在SM相似系数为0.74时可将35个试验品种划分成7个亚组,9份‘大久保’种质聚为一类,聚类结果与各品种间系谱关系基本吻合。在遗传相似系数为0.77的阈值时,9个‘大久保’被分成两类:来源于北京和易县的‘早久保’聚为一类,其它7个‘大久保’桃聚为一类,这与按照果实发育期以及成熟期对9个‘大久保’的分类结果一致。
In order to study the diversity of ’Okubo’ resources, genetic correlation analysis was carried out using 9 ’Okubo’ germplasm and 26 other peach germplasms from different sources by using SSR markers. The results of PCR amplification showed that 93 alleles were amplified by 13 SSR primers selected from 44 pairs of primers, of which 91 were polymorphic, accounting for 97.85%. The average number of polymorphic bands was 7 Article. The clustering results showed that when SM similarity coefficient was 0.74, 35 test cultivars could be divided into 7 subgroups, and 9 ’Okubo’ germplasms clustered together. The clustering results were in good agreement with the pedigree relationships among the cultivars. At the threshold of genetic similarity coefficient of 0.77, nine Okubo are divided into two categories: ’Okubo’ from Beijing and Yixian, and the other seven ’Okubo’ into one category, According to the fruit development and maturity of 9 ’Okubo’ classification results.