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Objective:The paper aimed to study the relationship between the expressions of immunoglobulin G(IgG) subclasses toward human papillomavirus 16-like particles(HPV16VLPs) in the serum of patients and different grades of cervical lesions.Methods:The expressions of IgG subclasses in 32 cases of human papillomavirus(HPV) infection,30 cervical intraepithelial neoplasia(CIN I),43 CIN Ⅱ-Ⅲ,and 24 hysteromyoma and chronic cervicitis were examined by ELISA.Results:The absorbance values of HPV16VLPs-IgG,IgG1 increased with the grade of CIN(P < 0.05).The IgG2 dominance(IgG2/IgG1 ratio > 1) from control group was 100%,87.50% for HPV infection group,75% for CIN I group,compared with that from CIN Ⅱ-Ⅲ patients(9.52%)(P < 0.05).The positive rates and absorbance values of HPV16VLPs-IgG,IgG1 and IgG2 from HPV16-DNA positive group were significantly higher than those from non-HPV16-DNA positive group(P < 0.05).There was a moderate correlation between the HPV16-DNA testing and detection of HPV16VLPs-IgG(r = 0.531,P < 0.05).Conclusion:An increase of the expressions of HPV16VLPs-IgG and its subclasses in the serum of the patients with cervical precancerous lesions,especially those with CIN II-III,might be associated with duration of HPV infection and severity of cervical lesions.An increase of the IgG2 dominance(IgG2/IgG1 > 1) in serum from low grade cervical lesions group and normal control group,might indicate the clearance of HPV infection and the regression of cervical lesions.
Objective: The paper aimed to study the relationship between the expressions of immunoglobulin G (IgG) subclasses toward human papillomavirus 16-like particles (HPV16 VLPs) in the serum of patients and different grades of cervical lesions. Methods: The expressions of IgG subclasses in 32 Results of The absorbance values of HPV16 VLPs-IgG, IgG1 increased with the grade (CIN I), 43 CIN II-III, and 24 hysteromyoma and chronic cervicitis were examined by ELISA. Results: The absorbance values of HPV16 VLPs- of CIN I group, compared with that from CIN II-III patients (P <0.05). The IgG2 dominance (IgG2 / IgG1 ratio> 1) from control group was 100%, 87.50% 9.52%) (P <0.05). The positive rates and absorbance values of HPV16 VLPs-IgG, IgG1 and IgG2 from HPV16-DNA positive group were significantly higher than those from non-HPV16-DNA positive group a moderate correlation between the HPV16-DNA testing and detection of HPV16 VLP Confocal: An increase of the expressions of HPV16 VLPs-IgG and its subclasses in the serum of the patients with cervical precancerous lesions, especially those with CIN II-III, might be associated with s-IgG (r = 0.531, duration of HPV infection and severity of cervical lesions. Ann increase of the IgG2 dominance (IgG2 / IgG1> 1) in serum from low grade cervical lesions group and normal control group, may indicate the clearance of HPV infection and the regression of cervical lesions.