论文部分内容阅读
本文应用裂解气相色谱－质谱（Pyro－GC／MS）法解析了人型结核杆菌H37Rv标准株的全细胞裂解产物，毛细管柱分离出40余个组分。人型结核杆菌菌体裂解主要产物为呈节律性出现的烯烃，系为该属菌丰富类脂中脂肪酸碎片形成，从正已烯到二十烯。组分含量最多的是十碳烯烃，同时还检出了一些含氮化合物吡咯、呋喃、吲哚、鸟甙等。本研究为裂解色谱法鉴定分支杆菌的工作提供了定性的参照。
In this study, the whole cell lysate of Mycobacterium tuberculosis H37Rv strain was analyzed by Pyro-GC / MS. More than 40 components were separated by capillary column. Mycobacterium tuberculosis cell lysate is the main product of rhythmic alkenes, Department of the bacteria rich in lipid fatty acid fragments formed from n-hexene to eicosene. The most abundant component is the ten-carbon olefin, but also detected some nitrogen compounds pyrrole, furan, indole, guanosine and so on. This study provides a qualitative reference for the identification of mycobacteria by pyrolysis chromatography.