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钙调蛋白(CaM)参与脑中多种细胞过程的调节,推测它与大脑的分化发育有关。本实验研究了鸡脑从胚胎早期(原基)至大脑成熟各发育阶段可溶部分的CaM及其调节酶——环核苷酸磷酸二酯酶(PDE)的含量或活力变化。未经孵育的鸡胚中检测不出CaM,在孵育3—14天的胚脑中CaM含量逐渐增加:总CaM增加了3倍,活性CaM增加了4倍;而且在鸡脑细胞分裂分化最活跃的期间(皮质和髓质形成期间,即孵育8—14天),活性CaM的增加尤为显著(增加近3倍)。提示CaM与脑细胞的分裂分化有关。PDE的活力出现晚于CaM5天,随着胚龄增加,不断上升,提示它与脑细胞的分裂分化无直接关系,可能与大脑的功能活动有关。本实验还研究了鸡脑发育期中可逆性钙结合蛋白的变化。
Calmodulin (CaM) is involved in the regulation of various cellular processes in the brain, suggesting that it is involved in the development of the brain. In this study, the content or activity of CaM and its regulatory enzyme, cyclic nucleotide phosphodiesterase (PDE), in the soluble portion of chicken brain during early embryo (primordia) to mature brain were studied. No CaM was detected in the non-incubated chicken embryos, and the CaM content gradually increased in the embryo brain incubated for 3-14 days: the total CaM increased 3-fold and the active CaM increased 4-fold; (Cortical and medulla formation, ie, 8-14 days of incubation), the increase in active CaM was particularly significant (nearly three-fold increase). Tip CaM and brain cell division related. PDE vitality later than CaM5 days, with the increase of embryonic age, rising, suggesting that it is not directly related to the division of brain cells, may be related to brain function and activity. The experiment also studied the development of chicken brain reversible calcium-binding protein changes.