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Halogen Occultation Experiment (HALOE)data are used to study the quasi-biennial variability of CH4 mixing ratio in the middle stratosphere. The results of EOF analysis indicate that quasi-biennial period is principal for the interannual variability of methane. Antisymmetry with respect to the equator is significant for the methane QBO by explaining 59.3% variance, while the symmetric component only explains about 30%. The antisymmetry is more significant in 10°-20° latitude than between 10°S and 10°N. Analyses of the vertical motions show that anomalies of annual cycle play an important role in the antisymmetric distributions. Whereas, the zonal wind QBO is still the most important dynamical effect for the interannual variability of CH4 between 10°S-10°N.