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采用聚合酶链反应－单链构象多态性分析（ＰＣＲ－ＳＳＣＰ）技术，对同一卵巢上皮性肿瘤石蜡包埋组织中ｐ１６基因（第二外显子）突变及人乳头状瘤病毒（ＨＰＶ）感染进行相关性研究。并与正常卵巢组织进行对照。结果，２８例卵巢上皮性肿瘤组织中ｐ１６基因突变１５例，突变率为５３．６％（１５／２８），其中７例伴有ＨＰＶ１６型或ＨＰＶ１８型感染，占突变率的４６．７％。在卵巢上皮性肿瘤组ＨＰＶ１６、１８ＤＮＡ阳性率为５３．６％（１５／２８），对照组ＨＰＶ１６、１８ＤＮＡ阳性率为５．６（１／１８），二者比较有显著性差异。提示：卵巢上皮性肿瘤中ｐ１６基因突变与ＨＰＶ１６、１８型感染有关。ＨＰＶ１６、１８型感染与卵巢上皮肿瘤密切相关
Polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP) was used to detect mutations in the p16 gene (exon 2) in paraffin-embedded tissues of the same epithelial ovarian tumor and human papilloma virus (HPV) Infections were studied for correlation. And compared with normal ovarian tissue. As a result, there were 15 cases of p16 gene mutations in 28 epithelial ovarian tumors, and the mutation rate was 53.6% (15/28). Among them, 7 cases were associated with HPV16 or HPV18 infection, accounting for 46.7% of the mutation rate. The positive rate of HPV16 and 18DNA in ovarian epithelial tumors was 53.6% (15/28). In the control group, the positive rate of HPV16 and 18DNA was 5.6 (1/18). There was a significant difference between the two groups. Tip: p16 gene mutations in ovarian epithelial tumors are associated with HPV 16 and 18 infections. HPV16,18 infection is closely related to ovarian epithelial tumors