论文部分内容阅读
１９９５年末和１９９６年初，各有１名缅甸儿童到我国云南省就医，临床诊断为脊髓灰质炎（脊所）。从患者的粪便体本中各分离到１株病毒，经血清学定型均为脊灰１型。经ＰＣＲ－ＲＦＬＰ法检测，两株病毒的酶切图谱完全一致，但与Ｓｄｂｉｎ１有明显差异，判断为野毒株。对相同片段的核苷酸序列测定结果肯定了这一判断。其核苷酸序列经ＰＨＹＬＩＰ软件分析，显示其基因型与我国境内曾经流行过的野毒株有明显差异，说明这两株野毒株是由缅甸传人中国的“输入野毒株”。
In late 1995 and early 1996, one Myanmar child each went to Yunnan for medical treatment and polio (ridge). A virus was isolated from the patient’s stool samples and all were serotyped with poliovirus type 1. By PCR-RFLP test, the two virus digestion maps exactly the same, but significant differences with Sdbin1, judged as wild strains. The same fragment of the nucleotide sequence determination of the results affirmed this judgment. The nucleotide sequence was analyzed by PHYLIP software, which showed that the genotypes were significantly different from the wild-type strains that had been prevalent in our country. This indicated that the two wild-type strains were “imported wild-type strains” from Myanmar to China.