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[目的]观察益髓颗粒对免疫性血小板减少性紫癜(ITP)小鼠脾淋巴细胞凋亡及Bcl-2、Bax蛋白表达的影响。[方法]40只Balb/c小鼠随机分为正常组、模型组、泼尼松治疗组和益髓颗粒治疗组,除正常组外,其余各组均隔日1次按100μl/20g腹腔注射1:4豚鼠抗小鼠血小板血清(GP-APS)建立ITP小鼠模型,于造模第8天开始,各组均按0.2mL/10g·d~(-1)体积灌胃,其中,正常组、模型组灌服生理盐水,泼尼松按0.0113mg/10g·d~(-1)灌胃,益髓颗粒按0.3g/10g·d~(-1)灌胃,连续8天后处死动物,无菌取脾,石蜡包埋,切片4μm,用原位末端标记(TUNEL)法检测脾淋巴细胞凋亡,免疫组化法检测Bcl-2、Bax蛋白表达。[结果]与正常组比较,模型组脾淋巴细胞凋亡减少,Bcl-2蛋白表达增多,Bax蛋白表达减少(P<0.05);与模型组比较,泼尼松及益髓颗粒组脾淋巴细胞凋亡增多,Bcl-2蛋白表达减少,Bax蛋白表达增多(P<0.05)。[结论]ITP小鼠存在免疫功能失调可能与淋巴细胞凋亡及Bcl-2、Bax蛋白表达异常相关。益髓颗粒可通过调节淋巴细胞凋亡及Bcl-2、Bax蛋白表达而对ITP免疫失调起调节作用。
[Objective] To observe the effect of Yisui Granule on the spleen lymphocyte apoptosis and the expression of Bcl-2 and Bax in immune-induced thrombocytopenic purpura (ITP) mice. [Method] Forty Balb / c mice were randomly divided into normal group, model group, prednisone treatment group and Yishen Granule treatment group. All the rats in the other groups were injected intraperitoneally with 100μl / : 4 guinea pig anti-mouse platelet serum (GP-APS) ITP mouse model established at the beginning of the 8th day of modeling, each group by 0.2mL / 10g · d -1 volume gavage, of which, the normal group The model group was fed with normal saline. Prednisone was given orally at a dose of 0.0113mg / 10g · d -1, and the propolis particles at the dose of 0.3g / 10g · d -1. The animals were sacrificed for 8 days. Apoptotic spleen was embedded in paraffin and sectioned 4μm. The apoptosis of splenic lymphocytes was detected by TUNEL method. The expressions of Bcl-2 and Bax protein were detected by immunohistochemistry. [Results] Compared with the normal group, the apoptosis of splenic lymphocytes decreased, the expression of Bcl-2 increased and the expression of Bax decreased in the model group (P <0.05). Compared with the model group, the prednisone and the benefit marrow group spleen lymphocytes Apoptosis increased, Bcl-2 protein expression decreased, Bax protein expression increased (P <0.05). [Conclusion] The immune dysfunction of ITP mice may be related to the apoptosis of lymphocytes and the abnormal expression of Bcl-2 and Bax protein. Yixian particles can regulate the lymphocyte apoptosis and Bcl-2, Bax protein expression of ITP immune disorders play a regulatory role.