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野鲮亚科(Labeoninae)隶属鲤形目(Cypriniformes)中的鲤科(Cyprinidae,约有26个属近300个种。为研究其在鲤科鱼类中的系统发育关系,以16S rRNA基因作为遗传标记进行鲤科鱼类的系统发育分析。通过PCR方法, 扩增了长度为361-362 bp的3种野鲮亚科鱼类的16S rRNA基因部分序列,序列比对得到364个排列位点,其中94个为信息位点,占全部位点的26.8%。用Mega 2.1软件的NJ法构建分子系统树,结果表明:野鲮亚科鱼类没有形成单系类群,野鲮亚科的鲮、角鱼与鲤亚科的鲤、鲫形成姐妹群,然后再与野鲮亚科的麦鲮和露斯塔野鲮聚在一起,说明鲮与鲤亚科鱼类具有较近的亲缘关系。
The genus Labeoninae belongs to Cyprinidae (Cypriniformes), about 26 species belong to nearly 300 species.To study its phylogenetic relationship in cyprinid fish, the 16S rRNA gene was used as Phylogenetic analysis of Cyprinid fishes using phylogenetic analysis.The 16S rRNA gene partial sequences of 363-362 bp in length from 361 to 362 bp were amplified by PCR, Of which 94 were informative sites, accounting for 26.8% of the total loci. The molecular phylogenetic tree was constructed using the NJ method of Mega 2.1 software. The results showed that: , Horn carp and common carp subfamily common carp, crucian carp form a sister group, and then with the wild Gracilis subfamily wheat and Lost tower wild Gather together, indicating that Galliflorus and Cyprinidae fish have a closer genetic relationship.