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用1株O157H7菌株和另外11株人类肠道主要病原细菌配制O157H7单菌疫苗和多菌联合多价疫苗,对荷斯坦牛进行免疫实验。对免疫常乳中抗EHEC O157H7特异性抗体凝集价进行了长期监测,并对受试奶牛的免疫反应、应激反应及疫苗对奶牛可能产生的毒性作用进行了观察。结果证明,疫苗免疫效果很好,所有奶牛在整个泌乳期一直保持较高的特异性抗体效价(滴度),均在27以上,下个泌乳期开始阶段也未见明显下降的迹象。本次实验意外发现约有30%的未进行过免疫注射的对照组乳样有较高的O157H7特异性抗体滴度,个别乳样凝集价高达27,这可能说明这些牛群中有较高的O157H7感染率或携带率。O157H7是人类近二十余年来才开始认识到的出血性结肠炎(HC)的主要致病菌,能造成出血性腹泻和其它严重并发症。是值得研究和重点防范的病原细菌。
One O157H7 strain and the other 11 strains of human enteric pathogens were used to prepare O157H7 single-bacteria vaccine and multi-bacteria combined multivalent vaccine to test the immunity of Holstein cattle. The long-term monitoring of the agglutination price of anti-EHEC O157H7-specific antibody in the immune regular milk was conducted. The immune response, stress response and the possible toxic effect of the vaccine on the dairy cow were also observed. The results showed that the vaccine had a good immunization effect, and all the cows maintained high specific antibody titers (titers) throughout the lactation period, all of which were above 27, with no obvious signs of decline at the beginning of the next lactation period. This experiment unexpectedly found that about 30% of the non-immunized control milk samples had higher specific antibody titers of O157H7, with individual milk samples having a high titered value of 27, which may indicate that the higher O157H7 infection rate or carrier rate. O157H7 is the main pathogen of hemorrhagic colitis (HC) that human beings have been aware of for more than two decades and can cause hemorrhagic diarrhea and other serious complications. It is worth to study and focus on prevention of pathogenic bacteria.