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以仔猪小肠上皮细胞为模型,应用实时荧光定量PCR方法测定相关基因指标,研究刺五加苷B(EB)对仔猪小肠上皮(IPEC-J2)细胞紧密连接蛋白及炎性细胞因子mRNA表达量的影响。结果表明:0.1 mg/m L刺五加苷B显著增强肠道细胞紧密连接蛋白Occludin、Claudin-3、ZO-1和抗炎性细胞因子IL-10和TGF-β的mRNA表达量,且减弱促炎性细胞因子IL-6、TNF-α、INF-γ的mRNA表达量。说明刺五加苷B可通过改变细胞间紧密连接蛋白和细胞因子的表达从而对维持肠道屏障功能的完整性起到促进作用,有益于仔猪肠道的健康发育。
The piglets intestinal epithelial cells were used as a model, and the related gene indexes were determined by real-time fluorescence quantitative PCR to study the expression of tight junction proteins and inflammatory cytokines in the small intestine epithelium of piglets (IPEC-J2) influences. The results showed that: 0.1 mg / mL acanthoside B significantly increased mRNA expression of claudin-4, Claudin-3, ZO-1 and anti-inflammatory cytokines IL-10 and TGF- The mRNA expression levels of proinflammatory cytokines IL-6, TNF-α and INF-γ. It shows that acanthopanax B can promote the integrity of intestinal barrier function by altering the expression of claudin and cytokines, which is beneficial to the healthy development of piglet intestine.