人类免疫缺陷症病毒(HIV,LAV/HTLV-Ⅲ)感染病人血清学诊断中,采用的酶联免疫吸附法(ELISA)、免疫荧光法(IF)、蛋白质印迹法(WB)和放射免疫沉淀法(RIPA)常常需要操作活病毒或病毒感染的细胞。作者用一种重组肽取代病毒,用重组肽的WB检测患者血清,比常规WB更敏感,可与ELISA媲美。用PEX-2和pCL 970作质粒载体。这两 Serological diagnosis of human immunodeficiency virus (HIV, LAV / HTLV-III) infection was performed by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), Western blotting (WB) and radioimmunoprecipitation (RIPA) often requires the handling of live or virus infected cells. The authors replaced the virus with a recombinant peptide that detected the serum of patients with recombinant peptide WB, which was more sensitive than conventional WB and comparable to ELISA. PEX-2 and pCL 970 were used as plasmid vectors. These two