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为探讨MAC亚溶破效应在移植肾慢性排斥反应发病机制中的作用和CD59的保护作用,建立了肾小球血管内皮细胞培养方法,并用酵母多糖(Z)激活血清(NHS)补体,诱生MAC损伤肾小球血管内皮细胞,以上清中LDH释放量及脱落细胞活力确定NHS的亚溶破损伤剂量。结果显示:用肾小球外植法培养肾小球血管内皮细胞较易成功,当NHS为0.05ml(5%)时,上清中的LDH和脱落细胞活力,与正常组比较相差显著(P<0.05)。
In order to explore the role of MAC sub-melting effect in the pathogenesis of chronic rejection in renal allograft and the protective effect of CD59, a culture method of glomerular endothelial cells was established and the complement of ZHS activated serum (NHS) was induced to induce MAC injury of glomerular endothelial cells, the supernatant LDH release and exfoliated cell activity to determine NHS sub-soluble damage dose. The results showed that glomerular endothelial cells cultured with glomerulus were easier to succeed. When the NHS was 0.05ml (5%), the activity of LDH and exfoliated cells in the supernatant was significantly different from that of the normal group P <0.05).