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目的:探讨维拉帕米抗肝纤维化作用的机制。方法:采用链霉蛋白酶、胶原酶及密度梯度离心的方法,分离大鼠肝脏贮脂细胞,用MTT方法,观察了维拉帕米对贮脂细胞增殖的影响。结果:低浓度维拉帕米的抑制作用不明显(P>0.05),当药物浓度上升为10~20μmol.L时,抑制作用增强,呈明显药物浓度依赖关系。结论:维拉帕米抗肝纤维化的作用是抑制了贮脂细胞的增殖
Objective: To investigate the mechanism of anti-hepatic fibrosis of verapamil. Methods: The rat liver fat-storing cells were isolated by pronase, collagenase and density gradient centrifugation. The effects of verapamil on the proliferation of lipid-storing cells were observed by MTT assay. Results: The inhibitory effect of verapamil at low concentration was not obvious (P> 0.05). When the drug concentration was increased to 10 ~ 20μmol. L, the inhibition was enhanced, showing a clear drug concentration-dependent relationship. Conclusion: The anti-hepatic fibrosis effect of verapamil inhibits the proliferation of lipid-storing cells