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目的：探讨维拉帕米抗肝纤维化作用的机制。方法：采用链霉蛋白酶、胶原酶及密度梯度离心的方法，分离大鼠肝脏贮脂细胞，用ＭＴＴ方法，观察了维拉帕米对贮脂细胞增殖的影响。结果：低浓度维拉帕米的抑制作用不明显（Ｐ＞０．０５），当药物浓度上升为１０～２０μｍｏｌ．Ｌ时，抑制作用增强，呈明显药物浓度依赖关系。结论：维拉帕米抗肝纤维化的作用是抑制了贮脂细胞的增殖
Objective: To investigate the mechanism of anti-hepatic fibrosis of verapamil. Methods: The rat liver fat-storing cells were isolated by pronase, collagenase and density gradient centrifugation. The effects of verapamil on the proliferation of lipid-storing cells were observed by MTT assay. Results: The inhibitory effect of verapamil at low concentration was not obvious (P> 0.05). When the drug concentration was increased to 10 ~ 20μmol. L, the inhibition was enhanced, showing a clear drug concentration-dependent relationship. Conclusion: The anti-hepatic fibrosis effect of verapamil inhibits the proliferation of lipid-storing cells