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目的:探讨中药海尔福口服液对铅中毒小鼠模型的排铅保健效果,为研究铅中毒儿童排铅保健作用机理及应用提供实验依据。方法:用乙酸铅水溶液行小鼠腹腔注射染毒,将染毒模型组分成不治疗组(即模型组)和治疗组,同时按大、小计量、又分成治疗1组和治疗2组。将正常小鼠设为对照组。治疗组用大小不同剂量口服液进行灌胃测定小鼠血红蛋白(Hb)。实验结束后,从眼球取血,分离血清,测定丙氨酸氨基转移酶(ALT)、肌酐、总蛋白含量,取肝、肾、睾丸送病理检查,取大脑做成10%脑匀桨,测定O2·清除率和脑铅含量。结果:对照组、模型组、治疗1组、治疗2组测定结果依次排列,造模前Hb各组比较差异无统计学意义(P>0.05);造模后(治疗前)Hb与对照组比较,差异有统计学意义(P<0.01);治疗后Hb对照组高于其他3组,差异有统计学意义(P<0.01),同时模型组低于治疗组(P<0.05或P<0.01);ALT,对照组低于其他3组,差异有统计学意义(P<0.01);肌酐,模型组高于其他3组(P<0.01);血清总蛋白,模型组低于对照组,差异有统计学意义(P<0.05);脑O2·清除率,与对照组比较,对照组清除率明显升高,差异有统计学意义(P<0.05或P<0.01);脑铅与模型组比较,差异有统计学意义(P<0.05或P<0.01);病理形态学研究表明,肝、肾、睾丸中毒组均有一定程度的损害,治疗后有明显好转。结论:实验表明,铅染毒小鼠后,使造血、肝、肾、睾丸组织细胞形态和功能及脑抗氧化功能等受到明显损害,治疗后通过排铅,使脑铅下降,损害有明显减轻,起到保健作用。
Objective: To investigate the effect of traditional Chinese medicine Haier Fu oral liquid on lead poisoning in mouse models of lead poisoning, and to provide experimental evidence for studying the mechanism and application of lead excretion health care in children with lead poisoning. Methods: The mice were injected intraperitoneally with lead acetate solution. The model group was divided into untreated group (model group) and treatment group. At the same time, the model group was divided into treatment group 1 and treatment group 2. Normal mice as a control group. The treatment group with different doses of oral liquid gavage mouse hemoglobin (Hb). At the end of the experiment, blood was taken from the eye and the serum was separated. The contents of alanine aminotransferase (ALT), creatinine and total protein were measured. The liver, kidney and testis were taken for pathological examination. The brain was made into 10% O2 · clearance and brain lead content. Results: The results of control group, model group, treatment group 1 and treatment group 2 were arranged in sequence, there was no significant difference in Hb before the model (P> 0.05); Hb in the control group , The difference was statistically significant (P <0.01); Hb control group after treatment was higher than the other three groups, the difference was statistically significant (P <0.01), while the model group was lower than the treatment group (P <0.05 or P <0.01) (P <0.01); creatinine, model group was higher than the other three groups (P <0.01); serum total protein, model group was lower than the control group, the difference was (P <0.05). Compared with the control group, the clearance rate of brain O2 was significantly increased in the control group (P <0.05 or P <0.01). Compared with the model group, The difference was statistically significant (P <0.05 or P <0.01). The pathomorphological studies showed that the liver, kidney and testicular poisoning groups all had a certain degree of damage, which was obviously improved after treatment. Conclusion: The experimental results show that lead poisoning can obviously damage the morphology and function of cells in hematopoietic, liver, kidney and testis, and the anti-oxidative function of brain. Lead excretion decreases and the damage is significantly reduced , Play a health role.