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Objective:To differentiate cryptic stage of Echinococcus granulosus(E.granulosus) and Taenia by PCR-RFLP and sequence information of amplicon.Methods:DNA were isolated from metacestodes stage of Taenia and E.granulosus using DNA isolation kit(Q-BIOgene kit,USA), the amplified and purified DNA product was then cloned and sent for sequencing.The generating sequence information was used for amplicons identification.Results:Out of 112 faecal and environmental samples,16 exhibited positive result.The product size of amplicon positive for E.granulosus was 310 bp;whereas,for Taenia spp.sizes varied from 379 to 388 bp.Restriction pr of ile of actinⅡwith Csp61 also differed Taenia spp.and E.granulosus.Conclusions:The result of the study indicated that,the primers were useful to differentiate cryptic stage of the two genera which is yet to be reported earlier.
Objective: To differentiate cryptic stage of Echinococcus granulosus (E.granulosus) and Taenia by PCR-RFLP and sequence information of amplicon. Methods: DNA were isolated from metacestodes stage of Taenia and E. ganulosus using DNA isolation kit (Q-BIOgene kit, USA), the amplified and purified DNA product was then cloned and sent for sequencing. The generating sequence information was used for amplicons identification. Results: Out of 112 faecal and environmental samples, 16 exhibited positive result. The product size of amplicon positive for E .granulosus was 310 bp; whereas, for Taenia spp.sizes varied from 379 to 388 bp. Restriction pr of ile of actinII with Csp61 also differed Taenia spp.and E.granulosus.Conclusions: The result of the study indicated that, the primers were useful to differentiate cryptic stage of the two genera which is yet to be reported earlier