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背景与目的生长抑素受体(somatostatin receptor,SSTR)作为基因的肿瘤标记物之一,正逐步受到研究者的重视。随着SSTR5种亚型先后被克隆,人们对SSTR家族各成员的氨基酸序列、分子生物学特性、在正常组织和肿瘤组织中的分布和表达及其特异性配体等方面的研究也进一步深入。本研究探讨SSTR亚型SSTR2A、SSTR5与表皮生长因子受体(epidermal growth factor receptor,EGFR)在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中的表达及其意义,并探讨其相关性。方法用免疫组化法(SP法)检测SSTR2A、SSTR5和EGFR三种蛋白在62例NSCLC组织和7例癌旁肺组织中的表达情况,并进行预后随访。结果在62例NSCLC组织中,SSTR2A蛋白阳性表达30例,占48.4%;SSTR5阳性表达44例,占71.0%。SSTR2A、SSTR5蛋白表达与NSCLC的TNM分期有密切关系(P<0.05),但与患者的年龄、性别、吸烟与否、病理类型、肿瘤大小和淋巴结转移均无明显关系(P>0.05)。EGFR蛋白在癌旁肺组织中无一例表达,而在NSCLC组织中有35例阳性,占56.5%。EGFR蛋白的表达与NSCLC患者的年龄、性别、吸烟与否、肿瘤组织类型、肿瘤大小、TNM分期、病理分级及淋巴结转移均无明显关系(P>0.05)。SSTR2A、SSTR5蛋白表达与EGFR蛋白的表达呈负相关关系。SSTR2A、SSTR5蛋白阳性表达者的3年生存率分别为64.52%和65.91%,阴性表达者为45.16%和22.22%,两者之间差异显著(P<0.05);EGFR蛋白阳性表达者的3年生存率为30.77%,阴性表达者为69.44%,两者之间差异显著(P<0.05)。结论NSCLC组织中SSTR和EGFR表达均显著增高,而且SSTR与EGFR的表达呈负相关,检测SSTR2A、SSTR5和EGFR表达对NSCLC淋巴结转移、病理分期及预后的评估有一定的临床意义。
Background and objective Somatostatin receptor (SSTR), one of the tumor markers of gene, is gradually receiving the attention of researchers. As SSTR5 subtypes have been cloned, the research on amino acid sequence, molecular biological characteristics, distribution and expression in normal tissues and tumor tissues, and specific ligands of SSTR family members has also been further studied. This study was designed to investigate the expression of SSTR2A, SSTR5 and epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC) Sex. Methods The expressions of SSTR2A, SSTR5 and EGFR in 62 NSCLC tissues and 7 adjacent non-cancerous lung tissues were detected by immunohistochemistry (SP method) and followed up for prognosis. Results Among the 62 cases of NSCLC, 30 cases were positive for SSTR2A protein (48.4%), while 44 cases were positive for SSTR5 (71.0%). The expression of SSTR2A and SSTR5 was closely related to the TNM staging of NSCLC (P <0.05), but not to age, gender, smoking, pathological type, tumor size and lymph node metastasis (P> 0.05). No EGFR protein was expressed in adjacent lung tissues, while 35 of them were positive in NSCLC tissues (56.5%). There was no significant correlation between the expression of EGFR protein and age, sex, smoking, tumor type, tumor size, TNM stage, pathological grade and lymph node metastasis in NSCLC patients (P> 0.05). SSTR2A, SSTR5 protein expression and EGFR protein expression was negatively correlated. The 3-year survival rates of SSTR2A and SSTR5 were 64.52% and 65.91%, respectively. The negative expression rates of SSTR2A and SSTR5 were 45.16% and 22.22%, respectively. There was significant difference between the two groups (P <0.05) The survival rate was 30.77%, while the negative expression was 69.44%. The difference between the two groups was significant (P <0.05). Conclusion The expressions of SSTR and EGFR in NSCLC tissues are significantly increased, and the expression of SSTR and EGFR is negatively correlated. It is of clinical significance to detect the expression of SSTR2A, SSTR5 and EGFR in lymph node metastasis, pathological stage and prognosis of NSCLC.