To evaluate laboratory germination media, seed germination rates and methodology for both Jatropha curcas and Pongamia pinnata were counted in different germination medium, such as river sand, quartz sand and vermiculite. The seeds of both species were sown at 2-cm depth of river sand, quartz sand and vermiculite. We evaluated methodologies of seeds of J. curcas and P. pinnata by ISTA(International Seed Testing Association). The results showed that river sand is the best medium for tree seeds planted at 2-cm depth of River sand. Vermiculite was second best. On paper media, there were many abnormal seedlings and this media were unsuitable for germination of the larger seeds. To evaluate laboratory germination media, seed germination rates and methodology for both Jatropha curcas and Pongamia pinnata were counted in different germination medium, such as river sand, quartz sand and vermiculite. The seeds of both species were sown at 2-cm depth of river sand , quartz sand and vermiculite. We evaluated methodologies of seeds of J. curcas and P. pinnata by ISTA (International Seed Testing Association). The results showed that river sand is the best medium for tree seeds planted at 2-cm depth of River sand . Vermiculite was second best. On paper media, there were many abnormal seedlings and this media were unsuitable for germination of the larger seeds.