论文部分内容阅读
[目的]建立蔷薇属植物的无菌快繁体系。[方法]以茎段为外植体,以10种蔷薇属植物为试验材料,在培养基中添加不同的同激素组合来建立其组织培养体系。[结果]10种蔷薇属植物在萌发培养基MS+0.5mg/LBA+0.01mg/LNAA上均可萌发,芽体健壮,萌发率可达到70%以上;10种蔷薇属植物各自适宜的增殖培养基略有不同,在增殖培养过程中,增殖率可以达到3.0%以上,丛生苗健壮,叶色正常;健壮小苗在添加0.1～0.2mg/LNAA的1/2MS培养基上诱导生根,根系生长良好,生根率可以达到90%～100%;将生根后的小苗驯化后移栽,成活率在95%左右。[结论]建立了蔷薇属植物的无菌快繁体系,为蔷薇属植物的分子育种奠定了基础。
[Objective] The research aimed to establish a sterile and rapid propagation system of rose plants. [Method] The stem segments were used as explants, 10 kinds of Rosa plants were used as experimental materials, and different hormone combinations were added into the medium to establish their tissue culture system. [Result] The ten species of Rosa could germinate on the germination medium MS + 0.5mg / LBA + 0.01mg / L NAA, the buds were robust and the germination rate could reach more than 70%. The 10 species of Rosa were suitable for their respective proliferation In the proliferation process, the proliferation rate could reach more than 3.0%, the seedling was robust and the leaf color was normal. The robust plantlet induced rooting on 1/2 MS medium supplemented with 0.1 ~ 0.2mg / L NAA, the root system grew well , The rooting rate can reach 90% ~ 100%; after rooting the young domesticated transplanted survival rate of about 95%. [Conclusion] The sterile rapid propagation system of Rosa was established, which lays the foundation for the molecular breeding of Rosa.