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由东乡野生稻的细胞悬浮培养物游离原生质体,采用改良的RY-2培养基以琼脂糖珠法进行包埋培养,能产生持续细胞分裂。预分化实验表明:ABA能有效地提高原生质体愈伤组织的再生能力。最后成功地诱导出原生质体再生植株。
The free protoplast from the cell suspension culture of Dongxiang Wild Rice was embedded and cultured by the agarose bead method using a modified RY-2 medium to produce sustained cell division. Pre-differentiation experiments show that: ABA can effectively improve the regenerative capacity of protoplast callus. Finally, protoplast regeneration plants were successfully induced.