【摘 要】
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The effect of lead, cadmium and zinc on thetranscriptions and structures of 5 DNA fragments was studied by RNAslot blot hybridization and the analyze of Restric
【机 构】
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Department of Biology
论文部分内容阅读
The effect of lead, cadmium and zinc on thetranscriptions and structures of 5 DNA fragments was studied by RNAslot blot hybridization and the analyze of Restriction FragmentLength Polymorphism (RFLP). The seeds of three wheat strains(Yunmai29, 1257, 5118) which had grown in contaminated area, HuizeLead-zinc mine, Yunnan Province of China for a long time and theuncontaminated area were taken as the experimental materials. Noobvious change of DNA structure was detected, but there were manydifferences in the DNA transcription levels. These results impliedthat lead, cadmium and zinc might inhibit DNA transcription and hadmuch more effect on gene expression than structure in wheat, wheatmight acclimate to metal pollution after having grown in pollutionarea for a long time and the interference of these metal ions ingene expression might be one of main mechanisms of metal toxicityand plant adaptation. The results also showed the microevolution ofwheat in Lead-zinc mine.
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