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在北京顺义地区番茄栽培温室中随机抽取了15份疑似感染番茄斑萎病毒(TSWV)的植株叶片和果实,利用TSWV试剂盒进行DAS-ELISA检测,结果均为阳性,表明其感染了TSWV。利用来自秘鲁番茄的抗TSWV基因Sw-5的共显性SCAR标记,对442份番茄材料进行分析,筛选出24份含有Sw-5的材料,其中4份为野生番茄,1份为杂交种,16份为高代育种自交系,3份为野生潘那利番茄渐渗系。4份野生番茄综合农艺性状较差,如果实小、产量低、品质和风味差等,不能直接应用于育种;其余20份含Sw-5的材料综合农艺性状较好,可作为番茄抗TSWV的育种材料。
Fifteen leaves and fruit of tomato plants infected with tomato wilt wilt virus (TSWV) were randomly selected from the tomato cultivation greenhouse in Shunyi, Beijing. The results of TSAV ELISA showed that they were infected with TSWV. Using the co-dominant SCAR marker of anti-TSWV gene Sw-5 from Peruvian tomato, we analyzed 442 tomato materials and screened 24 materials containing Sw-5, of which 4 were wild tomato and 1 was hybrid, 16 were inbred lines of high generation breeds and 3 were introgression lines of tomato in wild Panalware. Four wild tomatoes had poor agronomic characters, but could not be directly applied to breeding if the yield was small, the yield was low and the quality and flavor were poor. The other 20 agronomic characters with Sw-5 were better, which could be used as tomato anti-TSWV Breeding materials.