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目的研究环氧合酶-2(COX-2)在重症急性胰腺炎(SAP)大鼠肝组织中的表达,探讨COX-2在SAP肝脏损伤中的作用。方法以3.5%牛磺胆酸钠胰胆管逆行注射诱导大鼠SAP模型。随机分为对照组和SAP组,术后4、8、16及24h测定血清淀粉酶(AMY)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肿瘤坏死因子(TNF)-α水平和腹水AMY;光镜下观察胰腺和肝组织损伤情况;免疫组化方法检测COX-2在肝组织中的表达。结果SAP组各时间点血清AMY、ALT、AST、TNF-α及腹水AMY水平均较对照组明显升高,差异均有统计学意义(P<0.05),与胰腺和肝脏组织的病理学改变相一致。术后不同时相COX-2在肝组织中的表达阳性率明显高于对照组,差异均有统计学意义(P<0.05),COX-2表达与ALT(rs=0.949,P=0.039)、AST(rs=0.972,P=0.016)及血清AMY(rs=0.944,P=0.041)呈正相关。结论SAP时COX-2表达上调可能在SAP合并肝脏损伤中发挥重要作用。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) in the liver of severe acute pancreatitis (SAP) rats and to explore the role of COX-2 in the liver injury of SAP. Methods The rat SAP model was induced retrograde injection with 3.5% sodium taurocholate and pancreaticobiliary duct. The rats were randomly divided into control group and SAP group. Serum amylase (AMY), alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor (TNF) -alpha and ascites AMY were observed; the pancreas and liver tissue damage was observed under light microscope; the expression of COX-2 in liver tissue was detected by immunohistochemistry. Results The levels of serum AMY, ALT, AST, TNF-α and ascites AMY in SAP group were significantly higher than those in control group at each time point (P <0.05), but not with the changes of pathological changes in pancreatic and hepatic tissues Consistent. The positive rate of COX-2 expression in liver tissue at different time points after operation was significantly higher than that in control group (P <0.05). The expression of COX-2 was positively correlated with ALT (rs = 0.949, P = 0.039) AST (rs = 0.972, P = 0.016) and serum AMY (rs = 0.944, P = 0.041). Conclusion The up-regulation of COX-2 expression in SAP may play an important role in SAP complicated with liver injury.